Embryonic erythroid differentiation in the human leukemic cell line K562.

Embryonic erythroid differentiation in the human leukemic cell line K562.

K562 human leukemia cells synthesize embryonic hemoglobins after culture in the presence of hemin. We have rigorously identified these hemoglobins by globin chain analysis and peptide mapping. No adult hemoglobin could be detected, and beta-globin synthesis was less than 2 ppm of total protein synthesis. Persistent embryonic globin gene expression is known to occur as a consequence of globin ge...

Embryonic-fetal erythroid characteristics of a human leukemic cell line.

We have studied a number of cell surface, enzyme, and protein markers in the human leukemic K562 cell line. We have confirmed previous observations that these cells accumulate human embryonic hemoglobins after exposure to hemin. In addition, our results demonstrated that these cells possess in the "ininduced" state i surface antigen, lactate dehydrogenase isoenzymes characteristic of embryonic ...

Role of Phosphoramidate Antisense in Specific Inhibition of K562 Leukemic Cell Line

Butyrate-induced erythroid differentiation of human K562 leukemia cells

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Production and characterization of monoclonal antibodies against the human leukemic cell line K562.

The cell line K562 was originally established from a patient with chronic myeloid leukemia (CML) in terminal blast crisis [1]. The cells have the potential of self-renewal and pluripotency to differentiate into progenitors of different blood cells [2]. The aim of the present paper was to produce monoclonal antibodies which might be useful as indicators for the earliest differentiation stages of...